What question did this study set out to answer?

Identify the precise cleavage site and sequence determinants required for poliovirus 3C protease to cleave human TATA-binding protein (TBP).

June 1, 1993Journal of VirologyOpen Access

Identification of the cleavage site and determinants required for poliovirus 3CPro-catalyzed cleavage of human TATA-binding transcription factor TBP

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Identify the precise cleavage site and sequence determinants required for poliovirus 3C protease to cleave human TATA-binding protein (TBP).
Generated site-directed mutations at three candidate glutamine-glycine sites (amino acids 12, 18, and 108) in human TBP.
Introduced targeted amino acid substitutions at positions proximal to the cleavage site, including P4 and P2, to assess enzyme substrate specificity.
Poliovirus 3C protease selectively cleaved human TBP at the glutamine-glycine bond at position 18, leaving sites at positions 12 and 108 uncleaved.
Cleavage required both the glutamine and glycine residues at position 18, alongside specific proximal residues including alanine at P4 and proline at P2.

Structured PICO

Population

In vitro and in vivo models studying human TATA-binding protein (TBP) and poliovirus protease 3CPro

Intervention

Site-directed mutagenesis of TBP at glutamine-glycine sites (amino acids 12, 18, and 108) and surrounding residues

Comparator

Wild-type TBP and TBP with mutations at other sites

Outcome

Cleavage of TBP by viral protease 3CPro

The cleavage specificity of poliovirus 3CPro for the cellular transcription factor TBP closely mirrors its cleavage of viral polyproteins, specifically targeting the glutamine-glycine bond at position 18.

Discussion

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Implication

Findings from this animal study should not change practice; extends 3CPro substrate specificity to host TBP but leaves in vivo relevance open.

Cite This Study

Das et al. (1993) studied this question.

synapsesocial.com/papers/69d5722e75589c71d767e5ae https://doi.org/10.1128/jvi.67.6.3326-3331.1993

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Implication

Findings from this animal study should not change practice; extends 3CPro substrate specificity to host TBP but leaves in vivo relevance open.

Identification of the cleavage site and determinants required for poliovirus 3CPro-catalyzed cleavage of human TATA-binding transcription factor TBP

Key Points

Structured PICO

Discussion

Implication

Cite This Study

Identification of the cleavage site and determinants required for poliovirus 3CPro-catalyzed cleavage of human TATA-binding transcription factor TBP

Key Points

Structured PICO

Discussion

Implication

Cite This Study

Also Consider

Also Consider