MicroRNA-196a links human body fat distribution to adipose tissue extracellular matrix composition

Fig. 1. a. Volcano plot showing qPCR validation data of selected differentially expressed miRNAs between gluteal and abdominal subcutaneous adipose tissue (n = 40 paired biopsies). Positive log2 fold changes indicate higher expression in gluteal adipose tissue. Those that retained significance are indicated with name and their data is presented in Table S2. b. miR-196a expression in in vitro differentiated primary pre-adipocytes derived from ASAT and GSAT (n = 6; mean ± SE; *p < 0∙05, **p < 0∙005, paired t-test). c. miR-196a expression in in vitro differentiated imAPAD and imGPAD cell lines derived from ASAT and GSAT respectively (n = 6; mean ± SE; * p < 0∙05, paired t-test).

Fig. 2. a. miR-196a expression in paired ASAT and GSAT biopsies from age, sex and genotype-balanced group of rs11614913 (n = 30 in each group), **p < 0∙005; t-test. b. Folding and free energy of binding of miR-196a-2 containing the wild-type allele (top) and minor allele (bottom), as predicted in M-fold.

Fig. 3. a. Expression of miR-196a in imGPAD and imAPAD cell lines following transduction of miR-196aKD or control vector (*p < 0∙05 **p < 0∙005; paired t-test). b. miR-196a and HOXC8 protein in HEK293 cell lines following transduction of miR-196aKD or control vector (*p < 0∙05; paired t-test). c. Preadipocyte doubling time of the imGPAD and imAPAD cell lines following transduction of miR-196aKD or control vector, **p < 0∙005. d. Light microscopy (x10 magnification) of the imGPAD and imAPAD cell lines following transduction of miR-196aKD or control vector after 14 days of in vitro differentiation. e. mRNA expression of PPARγ2 and CEBPα throughout the 14-day differentiation time-course. f. COL25A1 expression in paired ASAT and GSAT biopsies from age, sex and genotype-balanced group of rs11614913 (n = 30 in each group).