What question did this study set out to answer?

To evaluate how variations in nanoparticle diameter and surface chemical functionalization govern the formation and identity of the long-lived protein corona formed in human plasma.

September 23, 2008Proceedings of the National Academy of SciencesOpen Access

Nanoparticle size and surface properties determine the protein corona with possible implications for biological impacts

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Authors

MLMartin Lundqvist JSJohannes Stigler GEGiuliano Elia

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Overview

In vitro study demonstrates that nanoparticle size and surface charge significantly alter protein corona composition in human plasma, highlighting the need for standardizing nanomaterial surfaces.

Key Points

To evaluate how variations in nanoparticle diameter and surface chemical functionalization govern the formation and identity of the long-lived protein corona formed in human plasma.
Tested six distinct polystyrene nanoparticles across three surface chemistries (plain unmodified, carboxyl-modified, and amine-modified) at two sizes (50 nm and 100 nm).
Incubated the nanoparticles in human plasma to isolate the long-lived ("hard") protein corona.
Identified and classified shared and unique adsorbed proteins according to their functional properties.
Both nanoparticle size (50 nm vs. 100 nm) and surface chemistry significantly dictated the specific protein makeup of the hard corona, even among identical core polystyrene materials.
Particles exhibited distinct distributions of conserved and unique proteins classified by biological function depending on diameter and surface charge modifications.

Cite This Study

Lundqvist et al. (2008) studied this question.

synapsesocial.com/papers/69d7c1043b601d7be3ae2b4c https://doi.org/10.1073/pnas.0805135105

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September 23, 2008Proceedings of the National Academy of SciencesOpen Access

Nanoparticle size and surface properties determine the protein corona with possible implications for biological impacts

View Full Paper

Ask AI

Bookmark

Authors

MLMartin Lundqvist JSJohannes Stigler GEGiuliano Elia

Discussion

Member takes

Overview

In vitro study demonstrates that nanoparticle size and surface charge significantly alter protein corona composition in human plasma, highlighting the need for standardizing nanomaterial surfaces.

Key Points

To evaluate how variations in nanoparticle diameter and surface chemical functionalization govern the formation and identity of the long-lived protein corona formed in human plasma.
Tested six distinct polystyrene nanoparticles across three surface chemistries (plain unmodified, carboxyl-modified, and amine-modified) at two sizes (50 nm and 100 nm).
Incubated the nanoparticles in human plasma to isolate the long-lived ("hard") protein corona.
Identified and classified shared and unique adsorbed proteins according to their functional properties.
Both nanoparticle size (50 nm vs. 100 nm) and surface chemistry significantly dictated the specific protein makeup of the hard corona, even among identical core polystyrene materials.
Particles exhibited distinct distributions of conserved and unique proteins classified by biological function depending on diameter and surface charge modifications.

Cite This Study

Lundqvist et al. (2008) studied this question.

synapsesocial.com/papers/69d7c1043b601d7be3ae2b4c https://doi.org/10.1073/pnas.0805135105

View Full Paper

Ask AI

Bookmark