Why the study?
Does an LC-MS/MS assay improve the measurement of plasma renin activity compared to RIA in human plasma samples?
Does an LC-MS/MS assay improve the measurement of plasma renin activity compared to RIA in human plasma samples?
A newly developed LC-MS/MS assay for plasma renin activity improves upon traditional RIA by reducing sample handling and identifying confounding Ang I degradation in clinical samples.
May warrant lab evaluation of LC-MS/MS for PRA; leaves open outcome validation in hypertension cohorts.
Our method requires reduced sample handling when compared with an RIA and eliminates the need for extended generation times for samples with low renin activity. Degradation of Ang I during generation appears to be a confounding variable in the interpretation of results from some clinical samples. Samples with profound degradation activity can be identified using a degradation standard that is added at the start of generation.
No takes yet. Share an insight, caveat, or question.
Bystrom et al. (2010) studied this question.
Synapse has enriched 5 closely related papers on similar clinical questions. Consider them for comparative context: