Synthesis and biological evaluation of heterocyclic 1,2,4-triazole scaffolds as promising pharmacological agents

The biological screening results reveal that the compounds T₅ (MICBS, EC = 24.7 µM, MICPA, CA = 12.3 µM) and T₁₇ (MICAN = 27.1 µM) exhibited potent antimicrobial activity as comparable to standards ciprofloxacin, amoxicillin (MICCipro = 18.1 µM, MICAmo = 17.1 µM) and fluconazole (MICFlu = 20.4 µM), respectively. The antioxidant evaluation showed that compounds T₂ (IC₅₀ = 34.83 µg/ml) and T₃ (IC₅₀ = 34.38 µg/ml) showed significant antioxidant activity and comparable to ascorbic acid (IC₅₀ = 35.44 µg/ml). Compounds T₃ (IC₅₀ = 54.01 µg/ml) was the most potent urease inhibitor amongst the synthesized compounds and compared to standard thiourea (IC₅₀ = 54.25 µg/ml). The most potent anticancer activity was shown by compounds T₂ (IC₅₀ = 3.84 μM) and T₇ (IC₅₀ = 3.25 μM) against HCT116 cell lines as compared to standard 5-FU (IC₅₀ = 25.36 μM).