August 9, 2017Scientific ReportsOpen Access

RNA sequencing of db/db mice liver identifies lncRNA H19 as a key regulator of gluconeogenesis and hepatic glucose output

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Population

Diabetic db/db mice, normal db/+ mice, HepG2 cells, and primary mouse hepatocytes

Comparison

Inhibition of lncRNA H19 using specific siRNA vs Normal db/+ mice and control cells

Design

Preclinical

Authors

NGNeha GoyalUniversity of Massachusetts Boston ASAmbily SivadasSt. John's National Academy of Health Sciences SVShamsudheen Karuthedath VellarikkalBroad Institute

Discussion

Member takes

Implication

H19 modulation merits preclinical follow-up in diabetes; animal data leave open human therapeutic relevance.

Structured PICO

Population

Diabetic db/db mice, normal db/+ mice, HepG2 cells, and primary mouse hepatocytes

Intervention

Inhibition of lncRNA H19 using specific siRNA

Comparator

Normal db/+ mice (for transcriptome profiling) and control cells (implied for siRNA experiments)

Outcome

Differentially expressed genes, gluconeogenic gene levels, and hepatic glucose outputsurrogate

The lncRNA H19 is a key regulator of hepatic glucose output and gluconeogenesis, and its downregulation in diabetes contributes to impaired glucose metabolism via FoxO1.

Cite This Study

Goyal et al. (2017) studied this question.

synapsesocial.com/papers/69d7efca3b601d7be3ae35f9 https://doi.org/10.1038/s41598-017-08281-7

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RNA sequencing of db/db mice liver identifies lncRNA H19 as a key regulator of gluconeogenesis and hepatic glucose output

Discussion

Member takes

Implication

Structured PICO

Cite This Study

RNA sequencing of db/db mice liver identifies lncRNA H19 as a key regulator of gluconeogenesis and hepatic glucose output

Discussion

Member takes

Implication

Structured PICO

Cite This Study

Also Consider

Also Consider