Performance and Mechanism of Fe₃O₄ Improving Biotransformation of Waste Activated Sludge into Liquid High-Value Products

This study demonstrated that Fe₃O₄ simultaneously improves the total production and formation rate of medium-chain fatty acids (MCFAs) and long-chain alcohols (LCAs) from waste activated sludge (WAS) in anaerobic fermentation. Results revealed that when Fe₃O₄ increased from 0 to 5 g/L, the maximal MCFA and LCA production increased significantly, and the optimal fermentation time was also remarkably shortened from 24 to 9 days. Moreover, Fe₃O₄ also enhanced WAS degradation, and the corresponding degradation rate in the fermentation system increased from 43.86 to 72.38% with an increase in Fe₃O₄ from 0 to 5 g/L. Further analysis showed that Fe₃O₄ promoted the microbe activities of all the bioprocesses (including hydrolysis, acidogenesis, and chain elongation processes) involved in the MCFA and LCA production from WAS. Microbial community analysis indicated that Fe₃O₄ increased the abundances of key microbes involved in abovementioned bioprocesses correspondingly. Mechanistic investigations showed that Fe₃O₄ increased the conductivity of the fermented sludge, providing a better conductive environment for the anaerobic microbes. The redox cycle of Fe(II) and Fe(III) existed in the fermentation system with Fe₃O₄, which was likely to act as electron shuttles to conduct electron transfer (ET) from the electron donor to the acceptor, thus increasing ET efficiency. This study provides an effective method for enhancing the biotransformation of WAS into high-value products, potentially bringing economic benefits to WAS treatment.