Biophysical study demonstrates measurement of reaction kinetics and diffusion in DNA–ethidium bromide systems, suggesting thermodynamic fluctuations can reveal coupled molecular mechanisms.
The temporal correlations of thermodynamic concentration fluctuations have been measured in a chemically reactive system at equilibrium by observing fluctuations of the fluorescence of a reaction product. The experiment yields the chemical rate constants and diffusion coefficients and shows the coupling among them. Data are reported for binding of ethidium bromide to DNA.
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Magde et al. (1972) studied this question.
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