Formate Oxidase (FOx) from Aspergillus oryzae: One Catalyst Enables Diverse H₂O₂‐Dependent Biocatalytic Oxidation Reactions

An increasing number of biocatalytic oxidation reactions rely on H₂ O₂ as a clean oxidant. The poor robustness of most enzymes towards H₂ O₂ , however, necessitates more efficient systems for in situ H₂ O₂ generation. In analogy to the well-known formate dehydrogenase to promote NADH-dependent reactions, we here propose employing formate oxidase (FOx) to promote H₂ O₂ -dependent enzymatic oxidation reactions. Even under non-optimised conditions, high turnover numbers for coupled FOx/peroxygenase catalysis were achieved.