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Nanopore sequencing of nucleic acids took nearly 30 years to develop and is now firmly established as an alternative to sequencing by synthesis methods Oxford Nanopore Technologies (ONT) released the first commercial nanopore device for DNA sequencing in 2014 and has continually improved the technology since then Although the read accuracy is only around 90%, ONT technology can sequence very long molecules and generates data in real time. In addition, RNA can be sequenced directly and modified bases can be detected
Léger et al. (Thu,) studied this question.