THP-1 and MCF-7 pair is a bioactivity detection sub-threshold system that is both efficient and sensitive as well as a highly effective preclinical test for dental material. The findings of this study confirm that orthodontic clear aligners, even after being trimmed on 3D-printed models, do not exhibit antigenic properties. This supports their continued use in intraoral applications where the absence of immunological reactivity is essential during the development phase.
Barbaro et al. (2026) studied this question.