Evaluation of Platelet Function Detection Using Sequential Platelet Counting Method via Fluorescent Platelet Channel

BACKGROUND: The sequential platelet counting method (SPCM) for platelet function detection relies on impedance-based platelet counting, which may be affected by factors such as microcytes and red blood cell fragments. The Sysmex XN series automated hematology analyzer incorporates a fluorescent platelet channel (PLT-F) based on flow cytometry, offering enhanced specificity compared to impedance methods. METHODS: This study compared platelet aggregation function between light transmission aggregometry (LTA) and SPCM-PLTF in healthy individuals and clopidogrel-treated patients. The effects of platelet-poor plasma (PPP) and normal saline (NS) dilution on LTA results were also analyzed. RESULTS: SPCM-PLTF revealed significant differences in platelet aggregation between healthy and patient groups (p < 0.0001). Although LTA and SPCM-PLTF results differed statistically (p < 0.0001), they exhibited strong correlation (r = 0.849, p < 0.0001). PPP dilution progressively reduced platelet aggregation (p < 0.05), while NS dilution showed no significant effect. CONCLUSIONS: SPCM-PLTF effectively monitors platelet function and correlates well with LTA, offering a viable alternative for clinical use.