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April 10, 2026Journal of Burn Care & ResearchOpen Access

C-754-01. Burn Injury Drives Biphasic Macrophage Immune Reprogramming Through Locus-Specific Epigenetic Remodeling

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Authors

HKHans KimRMRobert MaileAHAidan Higgs

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Overview

Experimental investigation shows biphasic immune response in mice post-burn injury, suggesting therapeutic targets.

Key Points

  • The aim is to understand how severe burn injury leads to immune dysfunction through macrophage reprogramming.
  • Performed full-thickness scald injury or sham procedure on female C57BL/6 mice.
  • Isolated splenic F4/80+ macrophages at specific time points post-injury.
  • Measured cytokine secretion in response to TLR stimulation.
  • Conducted transcriptomic profiling of over 1300 immune and metabolic genes.
  • Utilized MAPit-FENGC to assess DNA methylation and chromatin accessibility.
  • Macrophages exhibited a biphasic immune response with increased pro-inflammatory cytokines at day 2.
  • By day 14, there was a significant decrease in MCP-1 and TNFα levels with increased IL-10 secrtion.
  • Transcriptomic analysis revealed dynamic regulation of key immune genes.
  • Early chromatin accessibility at pro-inflammatory loci was noted, preceding changes in transcription.
  • Indicated a shift from hyperinflammation to immune tolerance through epigenetic mechanisms.

Cite This Study

Kim et al. (2026) studied this question.

synapsesocial.com/papers/69d895a86c1944d70ce06c49https://doi.org/10.1093/jbcr/irag033.029
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