Isotope Labeling in Insect Cells

This is the authors' abstract. We don't add key points for this paper.

Recent years have seen remarkable progress in applying nuclear magnetic resonance (NMR) spectroscopy to proteins that have traditionally been difficult to study due to issues with folding, posttranslational modification, and expression levels or combinations thereof. In particular, insect cells have proved useful in allowing large quantities of isotope-labeled, functional proteins to be obtained and purified to homogeneity, allowing study of their structures and dynamics by using NMR. Here, we provide protocols that have proven successful in such endeavors.

This methodology paper provides protocols for isotope labeling of proteins in insect cells, facilitating structural and dynamic studies using NMR spectroscopy.