Why the study?
Do published HRV-specific PCR primer pairs have sufficient sensitivity to detect all HRVs in clinical specimens?
Do published HRV-specific PCR primer pairs have sufficient sensitivity to detect all HRVs in clinical specimens?
A single published PCR primer pair is insufficient to detect all human rhinovirus strains, requiring multiple PCRs for accurate epidemiological description.
Single-primer HRV PCR misses strains in specimens; leaves open optimal multi-assay protocols for surveillance.
We conducted a preliminary comparison of the relative sensitivity of a cross-section of published human rhinovirus (HRV)-specific PCR primer pairs, varying the oligonucleotides and annealing temperature. None of the pairs could detect all HRVs in 2 panels of genotyped clinical specimens; >1 PCR is required for accurate description of HRV epidemiology.
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Faux et al. (2011) studied this question.
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