Preventing swarm detection in extracellular vesicle flow cytometry: a clinically applicable procedure

To prevent swarm detection in a series of clinical samples, the measurement count rate of a single diluted plasma sample can be used to determine the optimal dilution factor. For our samples, flow cytometer, and settings, the optimal dilution factor is ≥1.1 × 10²-fold, while the count rate is <1.1 × 10⁴ events·s⁻¹.