What question did this study set out to answer?

To quantify total phenolic contents and evaluate the spectral and fluorescence characteristics of Gul-e-Zoofa (Nepeta bracteata Benth) flowers for botanical identification and standardization.

January 13, 2017SHILAP Revista de lepidopterologíaOpen Access

Spectrophotometric determination of the total phenolic content, spectral and fluorescence study of the herbal Unani drug Gul-e-Zoofa ( Nepeta bracteata Benth)

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Authors

NSNadeem SiddiquiGlasgow Royal Infirmary ARAbdur RaufAbdul Wali Khan University Mardan ALAbdul LatifShaheed Benazir Bhutto University

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Implication

Laboratory analysis reveals high phenolic levels and distinct spectral profiles in Nepeta bracteata flowers, indicating antioxidant potential and quality control standards.

Key Points

To quantify total phenolic contents and evaluate the spectral and fluorescence characteristics of Gul-e-Zoofa (Nepeta bracteata Benth) flowers for botanical identification and standardization.
Quantified total phenolic content in ethanolic and aqueous extracts using spectrophotometry with the Folin-Ciocalteu reagent calibrated against gallic acid.
Conducted ultraviolet (UV) and infrared (IR) spectral analyses on the alcoholic flower extract to identify absorption peaks and molecular functional groups.
Evaluated fluorescence characteristics of powdered plant material and successive extracts under daylight and UV illumination both before and after chemical treatment.
Total phenolic content measured 326.28 mg/g gallic acid equivalent (GAE) in alcoholic extract and 319.14 mg/g GAE in aqueous extract.
Ultraviolet spectroscopy demonstrated a peak absorption wavelength at 320 nm in the alcoholic extract.
Infrared spectroscopy revealed characteristic functional group frequencies including 3465.31, 3220.07, 2927.3, 1709.07, and 1610.19 cm⁻¹ alongside specific powder fluorescence reactions.

Cite This Study

Siddiqui et al. (2017) studied this question.

synapsesocial.com/papers/69d901cc183921ebcaae460b https://doi.org/10.1016/j.jtumed.2016.11.006

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Implication

Laboratory analysis reveals high phenolic levels and distinct spectral profiles in Nepeta bracteata flowers, indicating antioxidant potential and quality control standards.

Spectrophotometric determination of the total phenolic content, spectral and fluorescence study of the herbal Unani drug Gul-e-Zoofa ( Nepeta bracteata Benth)

Discussion

Member takes

Implication

Key Points

Cite This Study

Spectrophotometric determination of the total phenolic content, spectral and fluorescence study of the herbal Unani drug Gul-e-Zoofa ( Nepeta bracteata Benth)

Discussion

Member takes

Implication

Key Points

Cite This Study

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