In vitro study demonstrates functional epithelial barrier formation in patient-derived intestinal organoid monolayers, suggesting utility for testing inflammatory bowel disease therapies.
We describe the generation and characterization of human colonic or ileal organoid models capable of functional differentiation to mature epithelial cell types. In monolayer culture, these cells formed a robust epithelial barrier with sustained TEER and responses to pharmacological modulation. Our findings demonstrate that control and IBD patient-derived organoids possess consistent transcriptional and functional profiles that can enable development of epithelial-targeted therapies.
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Jelinsky et al. (2022) studied this question.
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