Why the study?
Can a viral load threshold using quantitative real-time PCR distinguish symptomatic from asymptomatic rotavirus infection in children?
Can a viral load threshold using quantitative real-time PCR distinguish symptomatic from asymptomatic rotavirus infection in children?
A threshold cycle value of 26.7 on quantitative real-time PCR can distinguish symptomatic rotavirus gastroenteritis from asymptomatic infection in a high-burden African population.
May support qPCR threshold for rotavirus diagnosis in children; hypothesis-generating and requires prospective validation.
We evaluated quantitative real-time PCR to establish the diagnosis of rotavirus gastroenteritis in a high-disease-burden population in Malawi using enzyme immunoassay as the gold standard diagnostic test. In 146 children with acute gastroenteritis and 65 asymptomatic children, we defined a cutoff point in the threshold cycle value (26.7) that predicts rotavirus-attributable gastroenteritis in this population. These data will inform the evaluation of direct and indirect rotavirus vaccine effects in Africa.
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Bennett et al. (2015) studied this question.
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