The random amplified polymorphic DNA (RAPD) assay is a variant of the polymerase chain reaction (PCR) in which a single primer of random sequence is used at a low-annealing temperature. Twenty-two primers were evaluated for their usefulness in identifying 18 populations of Meloidogyne incognita, M. arenaria. M. javanica, and M. hapla collected worldwide. Reactions with primer OPA-01 produced amplified DNA bands whose size allowed the separation of the four species. Reactions with another group of 11 primers made possible the separation of one, two, or three species []
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José Luis Cenís (1993) studied this question.