Lipid-lowering effects of methanolic extract of Vernonia amygdalina leaves in rats fed on high cholesterol diet

We investigated the lipid-lowering effects of methanolic extract of Vernonia amygdalina (VA) leaves in rats fed an high cholesterol diet, and compared with a standard hypolipidemic drug, Questran (Qu).The effects of VA on the lipid profi le were assessed by measuring the levels of total cholesterol, triglyceride, low-density lipoprotein (LDL) cholesterol, high-density lipoprotein (HDL) cholesterol, lipid peroxidation (LPO), phospholipid, and glutathione (GSH) in the plasma and liver of the rats.Administration of cholesterol at a dose of 30 mg/0.3 ml, fi ve times in a week for nine consecutive weeks resulted in a signifi cant increase (p Ͻ 0.05) in plasma and post mitochondrial fraction (PMF) cholesterol levels by 33% and 55%, respectively.However, treatment with extract of VA at doses of 100 and 200 mg/kg caused a dose dependent reduction in the plasma and PMF cholesterol by 20%, 23% and 23%, 29%, respectively.Similar reduction in cholesterol levels was obtained in Qu-treated rats.Furthermore, VA at 200 mg/kg decreased the plasma and PMF LDL-cholesterol levels by 23% and 49%, and also decreased plasma and PMF triglyceride levels by 29% and 28%, respectively.Also, VA at 100 and 200 mg/kg caused a dosedependent increase in plasma HDL-cholesterol levels by 41% and 59%, respectively.However, there were no signifi cant differences (p Ͼ 0.05) in the PMF HDL-cholesterol and phospholipid levels of the treated rats when compared to hypercholesterolemic rats.There were signifi cant decreases (p Ͻ 0.05) in the LPO levels of extract-treated rats.Precisely, VA at 100 and 200 mg/kg decreased the levels of plasma and PMF LPO by 38%, 42% and 35%, 45%, respectively.In addition, VA augmented the cholesterol-induced decrease in PMF glutathione levels of the rats.Taken together, these results suggest the lipid-lowering effects of VA and, probably serve as a new potential natural product for the treatment of hyperlipidemia.