Ultrafiltration with size-exclusion liquid chromatography for high yield isolation of extracellular vesicles preserving intact biophysical and functional properties

Joel Z. Nordin; Yi Lee; Pieter Vader; Imre Mäger; Henrik J. Johansson; Wolf Heusermann; Oscar P. B. Wiklander; Mattias Hällbrink; Yiqi Seow; Jarred J. Bultema; Jonathan D. Gilthorpe; Tim Davies; Paul J. Fairchild; Susanne Gabrielsson; Nicole Meisner‐Kober; Janne Lehtiö; Smith Rjh; Matthew J. A. Wood; Samir EL Andaloussi

doi:10.1016/j.nano.2015.01.003

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Abstract

Recent evidence suggests extracellular vesicles (EVs) as another route of cellular communication. These EVs may be utilized for future therapeutics. In this article, the authors compared ultrafiltration with size-exclusion liquid chromatography (UF-LC) and ultra-centrifugation (UC) for EV recovery.

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Nordin et al. (2015) studied this question.

synapsesocial.com/papers/69d96a955e5bcb4e3b8365a6 https://doi.org/10.1016/j.nano.2015.01.003

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Ultrafiltration with size-exclusion liquid chromatography for high yield isolation of extracellular vesicles preserving intact biophysical and functional properties

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Abstract

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Ultrafiltration with size-exclusion liquid chromatography for high yield isolation of extracellular vesicles preserving intact biophysical and functional properties

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Abstract

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