What question did this study set out to answer?

To determine whether the nuclear protein spot 14 directly mediates the stimulation of hepatic lipid synthesis by thyroid hormone (T3) and glucose.

July 1, 1995Journal of Biological ChemistryOpen Access

Direct Evidence for a Role of the “Spot 14” Protein in the Regulation of Lipid Synthesis

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To determine whether the nuclear protein spot 14 directly mediates the stimulation of hepatic lipid synthesis by thyroid hormone (T3) and glucose.
Cultured primary rat hepatocytes under basal conditions (5.5 mM glucose without T3) and induction conditions (27.5 mM glucose and 50 nM T3).
Transfected hepatocytes with a spot 14 antisense oligonucleotide or a control oligonucleotide, with parallel testing in a spot 14-deficient rat hepatoma cell line.
Assayed lipogenesis, triglyceride formation, glucose uptake, and the expression and activity of key lipogenic enzymes (ATP-citrate lyase, fatty acid synthase, and malic enzyme).
Antisense transfection markedly inhibited spot 14 protein accumulation and completely prevented the T3- and glucose-induced increase in lipogenesis and triglyceride formation without affecting glucose uptake.
Knockdown of spot 14 inhibited the induction of ATP-citrate lyase and fatty acid synthase immunoreactivities, as well as malic enzyme activity and mRNA abundance.
Oligonucleotide treatment caused no significant alterations in lipogenesis in a control hepatoma cell line that naturally lacks spot 14 expression.

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Implication

In vitro study reveals that antisense inhibition of spot 14 prevents hormone- and glucose-stimulated lipogenesis in rat hepatocytes, indicating its direct role in lipid synthesis.

Cite This Study

Kinlaw et al. (1995) studied this question.

synapsesocial.com/papers/69d975cf00ab073a27836a3f https://doi.org/10.1074/jbc.270.28.16615

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Implication

In vitro study reveals that antisense inhibition of spot 14 prevents hormone- and glucose-stimulated lipogenesis in rat hepatocytes, indicating its direct role in lipid synthesis.

Direct Evidence for a Role of the “Spot 14” Protein in the Regulation of Lipid Synthesis

Key Points

Discussion

Implication

Cite This Study

Direct Evidence for a Role of the “Spot 14” Protein in the Regulation of Lipid Synthesis

Key Points

Discussion

Implication

Cite This Study

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