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Fidelity and efficiency. Nonribosomal peptide synthetases, which contain domains for the activation (A), thiolation (T), and condensation (C) of amino acids (AA), are high-efficiency, high-fidelity assembly lines for synthesizing peptide natural products. Errors in a single step can have serious consequences for product formation. Type II thioesterases (TEII) might play a critical role in ensuring efficiency and accuracy in these systems, essential features in any attempt to engineer NRPSs to produce novel products.
Yeh et al. (Thu,) studied this question.