The study identifies key molecular interactions of the TSWV NSm movement protein with viral N protein, RNA, and plant DnaJ family proteins, providing insights into its role in viral movement.
May guide antiviral target validation in plants; leaves open whether DnaJ interactions enable resistance breeding.
The nonstructural NSm protein of tomato spotted wilt tospovirus (TSWV) represents a putative viral movement protein involved in cell-to-cell movement of nonenveloped ribonucleocapsid structures. To study the molecular basis of NSm function, we expressed the protein in Escherichia coli and investigated protein-protein and protein-RNA interactions of NSm protein in vitro. NSm specifically interacts with TSWV N protein and binds single-stranded RNA in a sequence-nonspecific manner. Using NSm as a bait in a yeast two-hybrid screen, we identified two homologous NSm-binding proteins of the DnaJ family from Nicotiana tabacum and Arabidopsis thaliana.
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Soellick et al. (2000) studied this question.
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