Cysteine reactive chloroalkane probe enables HaloTag ligation for downstream chemical proteomics analysis.

Rubaba R Abanti; Dongqing Wu; Pavel Kielkowski

doi:10.1039/d6cb00004e

January 1, 2025RSC Chemical BiologyOpen Access

Cysteine reactive chloroalkane probe enables HaloTag ligation for downstream chemical proteomics analysis.

View Full Paper

Ask AI

Bookmark

Share

Key Points

The aim is to develop a chloroalkane probe for specific tagging of cysteine-containing proteins for proteomic analysis.
Synthesis of cysteine reactive chloroalkane probe.
Application of HALOtag technology for protein tagging.
Evaluation of probe specificity and reactivity in living cells.
The chloroalkane probe showed high specificity for cysteine residues.
Successful HALOtag ligation was achieved in a proteome-wide analysis.
Enhanced tracking of target proteins was observed, indicating effective tagging.

Implication

Chemical proteomics analyzes protein reactivity in living cells, suggesting enhanced tracking capabilities.

Abstract

Chemical proteomics is a powerful method to track proteins labelled by reactive small molecules in living cells on proteome-wide scale. The strategy relies on reactivity and specificity of bioorthogonal ‘click...

Discussion

No takes yet. Share an insight, caveat, or question.

Cite This Study

Abanti et al. (2025) studied this question.

synapsesocial.com/papers/69d9e52b78050d08c1b7575b https://doi.org/10.1039/d6cb00004e

View Full Paper

Ask AI

Bookmark

Share

Cysteine reactive chloroalkane probe enables HaloTag ligation for downstream chemical proteomics analysis.

Key Points

Implication

Abstract

Discussion

Cite This Study

Cysteine reactive chloroalkane probe enables HaloTag ligation for downstream chemical proteomics analysis.

Key Points

Implication

Abstract

Discussion

Cite This Study

Keep reading

Keep reading