In vitro study compares disinfectant effectiveness against Enterococcus faecalis, suggesting safer alternatives.
Aim: This study aimed to evaluate and compare the antimicrobial efficacy of Sodium Hypochlorite (NaOCl), Ethyl Alcohol, Triticum aestivum (Wheat Grass) + Saline, and T. aestivum + Ethyl Alcohol in disinfecting gutta percha (GP) points contaminated with Enterococcus faecalis . Methods: Forty-four GP cones were contaminated with E. faecalis and divided into four groups, each treated with one disinfectant for 5 min. Postdisinfection, GP cones were incubated in Brain–Heart Infusion broth and cultured on blood agar plates. Colony-forming units were counted and statistically analyzed using the one-way ANOVA test and post hoc Tukey’s test (P < 0.05). Results: T. aestivum + Ethyl Alcohol showed the highest antimicrobial activity, followed by T. aestivum + Saline and Ethyl Alcohol. NaOCl had the least effectiveness. Statistically significant differences were observed among all groups ( P < 0.05), rejecting the null hypothesis. Conclusion: T. aestivum combined with Ethyl Alcohol is an effective, safer alternative for chairside disinfection of GP cones, outperforming traditional agents such as NaOCl in antimicrobial efficacy against E. faecalis .
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Jain et al. (2026) studied this question.
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