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April 11, 2026Transfusion0 citations

Flow cytometry‐based detection of functional CCR5 variants: A scalable screening approach for HIV ‐resistant cord blood stem cells

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CSCarl SimardJPJosée PerreaultHéma-QuébecDFDiane FournierHéma-Québec

Key Points

  • The aim is to develop a flow cytometry-based method for identifying functional CCR5 variants in cord blood stem cells for HIV resistance.
  • Utilized a standardized, batch-normalized flow cytometry assay for screening
  • Focused on detecting reduced CCR5 expression in cord blood inventories
  • Emphasized a phenotype-first strategy to identify variants outside of CCR5Δ32
  • Successfully enriched for CCR5Δ32 and diverse non-Δ32 variants
  • Identified candidate HIV-resistant cord blood units that are not detected by Δ32-focused genotyping approaches
  • Demonstrated practicality for screening large inventories of cord blood stem cells

Abstract

A standardized, batch-normalized flow cytometry assay enables practical phenotypic screening of CBB inventories for reduced CCR5 expression and enriches for both CCR5Δ32 and diverse non-Δ32 variants. This phenotype-first strategy may complement targeted genotyping by identifying candidate HIV-resistant CBUs missed by Δ32-focused approaches.

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Cite This Study

Simard et al. (2026) studied this question.

synapsesocial.com/papers/69d9e67a78050d08c1b76d3dhttps://doi.org/10.1111/trf.70218
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