What question did this study set out to answer?

To evaluate whether the hydrophobic NH2-terminal 29 amino acids of cytochrome P450 2C1 independently mediate retention within the endoplasmic reticulum.

September 1, 1993Journal of Biological ChemistryOpen Access

The amino-terminal 29 amino acids of cytochrome P450 2C1 are sufficient for retention in the endoplasmic reticulum.

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Authors

KAKwangseog AhnSeoul National University ESElzbieta Szczesna-SkorupaUniversity of Illinois Urbana-Champaign BKB. KemperScripps Research Institute

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Implication

Experimental study demonstrates that the N-terminal 29 amino acids of cytochrome P450 2C1 direct ER retention in COS1 cells, suggesting an autonomous retention signal.

Key Points

To evaluate whether the hydrophobic NH2-terminal 29 amino acids of cytochrome P450 2C1 independently mediate retention within the endoplasmic reticulum.
Fused the NH2-terminal 29 amino acids of cytochrome P450 2C1 to E. coli beta-galactosidase and alkaline phosphatase reporter proteins.
Expressed hybrid constructs in COS1 cells and analyzed protein localization using subcellular fractionation, immunofluorescent microscopy, and endoglycosidase H sensitivity assays.
Both beta-galactosidase and alkaline phosphatase hybrid proteins cosedimented with endoplasmic reticulum membrane markers and exhibited distinct ER immunofluorescent staining patterns.
Carbohydrate moieties on glycosylated hybrid proteins exhibited sensitivity to endoglycosidase H digestion, confirming that the NH2-terminal 29 amino acids are sufficient for endoplasmic reticulum retention without the cytoplasmic domain.

Cite This Study

Ahn et al. (1993) studied this question.

synapsesocial.com/papers/69da1fc78988aeabbe6867d3 https://doi.org/10.1016/s0021-9258(17)46690-7

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Implication

Experimental study demonstrates that the N-terminal 29 amino acids of cytochrome P450 2C1 direct ER retention in COS1 cells, suggesting an autonomous retention signal.

The amino-terminal 29 amino acids of cytochrome P450 2C1 are sufficient for retention in the endoplasmic reticulum.

Discussion

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Implication

Key Points

Cite This Study

The amino-terminal 29 amino acids of cytochrome P450 2C1 are sufficient for retention in the endoplasmic reticulum.

Discussion

Member takes

Implication

Key Points

Cite This Study

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