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April 16, 2021PROTEOMICSOpen Access

Proteome characterisation of extracellular vesicles isolated from heart

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Why the study?

Isolation of cardiac extracellular vesicles remains a technical challenge, hindering the understanding of their protein composition despite their role as mediators of cardiac intercellular communication.

Population

Mouse heart tissue

Comparison

Langendorff-collagenase enzymatic perfusion and differential centrifugation vs mechanical isolation

Design

Preclinical laboratory study

Authors

BCBethany ClaridgeARAlin RaiHFHaoyun Fang

Discussion

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Overview

Does not inform clinical care; leaves open cEV roles in human cardiac disease pending validation.

Structured PICO

P
Population
Mouse heart tissue
I
Intervention
Langendorff-collagenase-based enzymatic perfusion and differential centrifugation for cardiac extracellular vesicle (cEV) isolation
C
Comparator
Mechanically-derived EVs
O
Outcome
Proteomic profiling and characterization of cardiac extracellular vesicles (cEVs)surrogate

A novel enzymatic perfusion method successfully isolates high-quality cardiac extracellular vesicles from mouse hearts, revealing a complex proteome indicative of diverse cellular origins and specialized functions.

Cite This Study

Claridge et al. (2021) studied this question.

synapsesocial.com/papers/69da25b58988aeabbe6869e2https://doi.org/10.1002/pmic.202100026
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