What question did this study set out to answer?

To investigate whether insulin-mediated vasodilation in human skeletal muscle vasculature is driven by endothelium-derived nitric oxide.

September 1, 1994Journal of Clinical InvestigationOpen Access

Insulin-mediated skeletal muscle vasodilation is nitric oxide dependent. A novel action of insulin to increase nitric oxide release.

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Why the study?

Is insulin-mediated skeletal muscle vasodilation dependent on endothelium-derived nitric oxide in normal subjects?

Population

Normal subjects

Comparison

N-monomethyl-L-arginine administered directly… vs Saline infusion (NS)

Design

Other

Authors

HSHelmut O. SteinbergVascular Medicine GBGinger BrechtelRichard L. Roudebush VA Medical Center AJAndi JohnsonLondon Studio Centre

Discussion

Member takes

Implication

May link insulin to nitric oxide-dependent vasodilation; leaves open relevance to insulin resistance states.

Key Points

To investigate whether insulin-mediated vasodilation in human skeletal muscle vasculature is driven by endothelium-derived nitric oxide.
Infused the nitric oxide synthase inhibitor L-NMMA (16 mg/min) directly into the femoral artery of normal subjects during normal saline infusion or a euglycemic hyperinsulinemic clamp.
Evaluated leg blood flow responses to graded intrafemoral infusions of methacholine chloride (endothelium-dependent) and sodium nitroprusside (endothelium-independent) during saline and hyperinsulinemic conditions.
Intrafemoral L-NMMA reduced leg blood flow from 0.296 ± 0.032 to 0.235 ± 0.022 L/min during saline and from 0.479 ± 0.118 to 0.266 ± 0.052 L/min during hyperinsulinemia (P < 0.03), raising NO-dependent flow from ~20% to ~40% (P < 0.003).
Leg blood flow increases to endothelium-dependent methacholine chloride were significantly augmented during hyperinsulinemia compared to saline (P < 0.03), whereas responses to sodium nitroprusside were not augmented.

Structured PICO

Is insulin-mediated skeletal muscle vasodilation dependent on endothelium-derived nitric oxide in normal subjects?

Population

Normal subjects

Intervention

N-monomethyl-L-arginine (L-NMMA) administered directly into the femoral artery at 16 mg/min during a euglycemic hyperinsulinemic clamp (HIC); graded intrafemoral artery infusions of methacholine chloride (MCh) or sodium nitroprusside (SNP)

Comparator

Saline infusion (NS)

Outcome

Leg blood flow (LBF)surrogate

Insulin-mediated vasodilation in skeletal muscle is dependent on endothelium-derived nitric oxide, suggesting insulin acts as a novel modulator of the EDNO system.

Cite This Study

Steinberg et al. (1994) studied this question.

synapsesocial.com/papers/69db23cf1e19c8ae08836ac0 https://doi.org/10.1172/jci117433

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Insulin-mediated skeletal muscle vasodilation is nitric oxide dependent. A novel action of insulin to increase nitric oxide release.

Discussion

Member takes

Implication

Key Points

Structured PICO

Cite This Study

Insulin-mediated skeletal muscle vasodilation is nitric oxide dependent. A novel action of insulin to increase nitric oxide release.

Discussion

Member takes

Implication

Key Points

Structured PICO

Cite This Study

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