Visualization of the internal ultrastructure of lichens using cross-sectional polishing SEM

January 1, 2025PLANT MORPHOLOGYOpen Access

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Key Points

The aim is to visualize the internal ultrastructure of lichens using an innovative SEM technique.
Fixed and embedded lichen-inhabited stones in epoxy resin.
Cut resin blocks using a diamond band saw and polished the surface.
Utilized field emission SEM for imaging the fine structures.
Observed microstructures of reproductive organs, symbiotic algae, and fungi within lichens.
Successfully visualized the cross-sectional microstructure of lichen reproducing organs.
Captured fine details of symbiotic algae and fungi.
Demonstrated effectiveness of cross-sectional polishing SEM for previously challenging samples.

This research demonstrates internal ultrastructure visualization of lichens using SEM, suggesting advancements in microscopic analysis techniques.

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Toyooka et al. (2025) studied this question.

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