The precise determination of highest occupied molecular orbital (HOMO) and lowest unoccupied molecular orbital (LUMO) energy levels is critical for understanding the photophysical and photochemical properties of carbon dots (C-dots), which directly govern their performance in optoelectronic, catalytic, and sensing applications. However, the lack of distinct redox peaks in cyclic voltammetry (CV) curves of C-dots poses a major challenge to conventional energy level calculation methods. Herein, we propose a novel strategy to calculate the HOMO–LUMO energy levels of C-dots by combining electron transfer (ET) kinetics with Marcus theory. A series of quinones (electron acceptors, EAs) and ferrocene derivatives (electron donors, EDs) were employed to quench the fluorescence of C-dots, and the ET rate constants (K) were derived from fluorescence lifetime measurements. The CV curves of EAs and EDs provided their respective oxidation and reduction potentials, which were used as reference energy levels. The UV–Vis absorption spectra confirmed that the fluorescence quenching mechanism was dominated by ET rather than energy transfer. Based on Marcus theory, the free energy change (ΔG) of ET reactions was correlated with K, and the HOMO and LUMO energy levels of C-dots were calculated to be −1.84 V (vs. SCE) and +1.60 V (vs. SCE), respectively. This study not only provides a reliable method for determining the energy levels of C-dots without distinct redox peaks but also deepens the understanding of ET mechanisms between C-dots and small molecules. The proposed strategy is expected to be extended to other fluorescent nanomaterials with similar CV limitations.
Yang et al. (Thu,) studied this question.