Effect of surface conditioning with cellular extracts on Escherichia coli adhesion and initial biofilm formation

Bacterial adhesion and subsequent biofilm formation start with surface conditioning by originating from the surrounding medium and from cell lysis. Different cell e.g. total cell extract (TCE), cytoplasm with cellular debris (CCDE) and periplasmic extract (PE) were tested in agitated 96-well microtiter plates and in a flow cell. Crystal assay demonstrated that a polystyrene substratum conditioned with TCE or CCDE initial biofilm formation, however cell adhesion generally increased when PE was. These results were dependent on conditioning film concentration. Using a parallel flow chamber, the use of optimal conditioning film concentrations resulted in all the cellular extracts reducing biofilm formation. Multifractal analysis was used to generate quantitative data on the number of cell clusters. Surface conditioning with cellular affected the amount and clustering of bacteria on polystyrene surfaces and propensity to induce biofilm formation. To the best of our knowledge, this is the first addressing the effect of cellular surface conditioning of cellular compartments on coli adhesion and initial biofilm formation. This work leads to a greater understanding of the factors that influence biofilm formation under flow conditions which are in food industry.(c) 2017 Institution of Chemical Engineers. Published by Elsevier B.V. All rights reserved