Pseudomonas aeruginosa is a high-priority pathogen responsible for up to 23% of infections in intensive care units, yet current diagnostic methods remain hindered by multiday delays or high technical complexity. In this study, we report a rapid and highly sensitive biosensor based on nanoporous anodic alumina (NAA) capped with a specific oligonucleotide molecular gate targeting the phzA2 gene. The S3 biosensor achieved a limit of detection (LOD) of 0.153 ng μL-1 for genomic DNA and an exceptional 28 CFU mL-1 for direct bacterial detection without the need for prior DNA extraction. Validation in 63 clinical urine samples demonstrated a sensitivity of 91.67% and a specificity of 94.87% (AUC: 0.961, p-value P. aeruginosa infections.
Torres-Mesado et al. (Fri,) studied this question.