A monoclonal culture of Emiliania huxleyi (PML92/11) was grown in natural sea-water under varying nitrate concentrations, ranging from 10 to 890μmol L -1 . Growth rate, calcification rate, particulate organic carbon pro- duction, particulate organic nitrogen production, and the ratio of aberrant coccoliths to normal coccoliths were deter- mined. None of these parameters showed a trend over the range of nitrate concentrations tested. It is concluded that high nitrate concentrations, typical for culture media like f/2, do not affect morphogenesis of coccoliths, nor do they affect the production of particulate organic carbon or nitrogen in this strain.
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Langer et al. (2009) studied this question.
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