Lymphopenia and risk of infection and infection-related death in 98,344 individuals from a prospective Danish population-based study

Fig 1. Flow chart of individuals from the Copenhagen General Population Study.

Fig 2. Regression toward the mean of lymphocyte counts, categorized as lymphopenia (<1.1 × 109/l), reference lymphocyte count (1.1–3.7 × 109/l), and lymphocytosis (>3.7 × 109/l) at the date of examination in 2003 in the Copenhagen General Population Study. When categorizing lymphocyte counts, lymphopenia was defined as a lymphocyte count below the 2.5th percentile, the reference category was defined as a lymphocyte count between the 2.5th and 97.5th percentile, and lymphocytosis was defined as a lymphocyte count above the 97.5th percentile. Median values and interquartile ranges of 5,181 individuals at the 2003 examination (left) and the same individuals—maintaining the 2003 categorization—at the 2013 examination (right). Regression dilution ratio is 2.04/2.98 = 0.68. The table below the graph gives the percentage of individuals in each lymphocyte category at the 2003 examination (rows) and at the 2013 examination (columns).

Fig 3. Risk of any infection as a function of lymphocyte count for individuals from the Copenhagen General Population Study. Solid red lines are hazard ratios, and dashed black lines indicate 95% confidence intervals based on fitting of cubic splines to risk estimates obtained using Cox proportional hazards regression. Multivariable adjustment includes all covariates listed in Table 1 except age, but with age as the underlying timescale. The median lymphocyte value of 2.1 × 109/l was set as reference for the continuous model. For the multivariable adjusted model, the lymphocyte interval 30–40 × 109/l is added to illustrate that for very high lymphocyte counts the risk of infections increases, but with a broad confidence interval. When categorizing lymphocyte counts, lymphopenia was defined as a lymphocyte count below the 2.5th percentile, the reference category was defined as a lymphocyte count between the 2.5th and 97.5th percentile, and lymphocytosis was defined as a lymphocyte count above the 97.5th percentile.

Fig 4. Multivariable adjusted risks of specific infections as a function of lymphocyte count for individuals from the Copenhagen General Population Study. Solid red lines are multivariable adjusted hazard ratios, and dashed black lines indicate 95% confidence intervals based on fitting of cubic splines to risk estimates obtained using Cox proportional hazards regression. Multivariable adjustment includes all covariates listed in Table 1 except age, but with age as the underlying timescale. The median lymphocyte value of 2.1 × 109/l was set as reference for the continuous model. The sum of the numbers of cases of specific infections exceeds the number of cases of “any infection” since individuals could have more than 1 specific infection. When categorizing lymphocyte counts, lymphopenia was defined as a lymphocyte count below the 2.5th percentile, the reference category was defined as a lymphocyte count between the 2.5th and 97.5th percentile, and lymphocytosis was defined as a lymphocyte count above the 97.5th percentile.

Fig 5. Multivariable adjusted risk of any infection for individuals from the Copenhagen General Population Study with lymphopenia (lymphocyte count < 1.1 × 109/l) compared to individuals with lymphocytes in the reference range (1.1–3.7 × 109/l). Multivariable adjustment includes all covariates listed in Table 1 except age, but with age as the underlying timescale. The sum number of individuals in the strata varies slightly because of varying numbers of individuals with missing data in the covariates. When categorizing lymphocyte counts, lymphopenia was defined as a lymphocyte count below the 2.5th percentile, while the reference category was defined as a lymphocyte count between the 2.5th and 97.5th percentile. NSNot significant after adjusting for 12 multiple comparisons using the Bonferroni method.aAccording to a likelihood ratio test.b≤168 versus >168 g/week for men and ≤84 versus >84 g/week for women.cWhen categorizing neutrophil counts, neutropenia was defined as a neutrophil count below 1.5 × 109/l, which is the generally accepted lower cutoff [4,41–43], the reference category was defined as a neutrophil count from 1.5 × 109/l to 5.9 × 109/l, and neutrocytosis was defined as a…

Fig 6. Multivariable adjusted risk of any infection for individuals from the Copenhagen General Population Study with lymphopenia using age-adjusted percentiles of lymphocyte count. Multivariable adjusted risk of any infection for individuals from the Copenhagen General Population Study with lymphopenia (lymphocyte count < 1.1 × 109/l) compared to individuals with lymphocytes in the reference range (1.1–3.7 × 109/l). Multivariable adjustment includes all covariates listed in Table 1 except age, but with age as the underlying timescale. Individuals with lymphopenia had a lymphocyte count below the 2.5th percentile in each 1-year age band. aAccording to a likelihood ratio test.

Fig 7. Multivariable adjusted risk of any infection for individuals from the Copenhagen General Population Study with lymphopenia stratified by follow-up time. Multivariable adjusted risk of any infection for individuals from the Copenhagen General Population Study with lymphopenia (lymphocyte count < 1.1 × 109/l) compared to individuals with lymphocyte count in the reference range (1.1–3.7 × 109/l). Multivariable adjustment includes all covariates listed in Table 1 except age, but with age as the underlying timescale. Follow-up time was segmented into 3 time intervals, and each individual was included in more than 1 time interval if total follow-up was above 2 years. When categorizing lymphocyte counts, lymphopenia was defined as a lymphocyte count below the 2.5th percentile, while the reference category was defined as a lymphocyte count between the 2.5th and 97.5th percentile. aAccording to a likelihood ratio test.

Fig 8. Algorithm. Absolute 2-year risks of hospitalization due to infection for different blood lymphocyte categories, stratified for age, sex, and smoking status. Nonsmokers were former smokers and never-smokers combined. Individuals diagnosed with immunodeficiency, hematologic disease, and/or autoimmune disease were not included when calculating absolute risks for the algorithm, since these individuals have a well-known high risk of infection. Numbers indicate absolute 2-year risk as rounded percentages.

Fig 9. Multivariable adjusted risk of infection-related death as a function of lymphocyte count for individuals from the Copenhagen General Population study. The solid red line is the multivariable adjusted hazard ratio, and black dashed lines indicate the 95% confidence interval based on fitting of cubic splines to risk estimates obtained using Cox proportional hazards regression. Multivariable adjustment includes all covariates listed in Table 1 except age, but with age as the underlying timescale. The median lymphocyte value of 2.1 × 109/l was set as reference for the continuous model. When categorizing lymphocyte counts, lymphopenia was defined as a lymphocyte count below the 2.5th percentile, the reference category was defined as a lymphocyte count between the 2.5th and 97.5th percentile, and lymphocytosis was defined as a lymphocyte count above the 97.5th percentile.