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In article number 1802384, Miguel Tovar, Marc Thilo Figge, and co-workers encode a population of microfluidic pico-liter droplets with colored polystyrene beads for the simultaneous study of multiple experimental conditions. Droplets are imaged using brightfield microscopy and are successfully decoded and evaluated by machine learning supported image analysis. The novel encoding strategy is applied to antibiotic susceptibility testing of droplet-encapsulated E. coli bacteria as a proof-of-principle.
Svensson et al. (Tue,) studied this question.