Closed vitrification based on DMSO and EG preserves the ultrastructural features and the ability to respond to the Ca²⁺ ionophore A23187 significantly better than does slow freezing with 0.3 M sucrose. Damage to organelles involved in the [Ca²⁺](i) modulation might reduce the developmental competence of cryopreserved oocytes.
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Gualtieri et al. (2011) studied this question.
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