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Being a unique biocatalyst to hydrolyse structural polysaccharides on the cell wall of bacteria, lysozyme can slow down bacterial resistance caused by antibiotic overdose treatments, however, efficient activation of lysozyme activities under pathogenic microenvironments remains challenging. Herein, a bio-interface engineering strategy was proposed for the remote modulation of thermoresistant lysozyme upon rationally designed photothermal nanoplatforms. For this, Ti3C2TX MXene nanosheets were functionalized by polydopamine (PDA) surface chemistry to enhance photothermal effects and performance durability, during which lysozyme biomacromolecules were immobilized at such a two-dimensional hybrid interface via intermolecular electrostatic affinity. The integrated nanoplatform (denoted as M@P@Lyso), with an optimal high light-to-heat conversion efficiency of 46.88%, realized not only precision control of local heat but also photo-responsive up-regulation for bio-catalysis of laden lysozyme. As a result, in vitro and in vivo antibacterial experiments revealed that M@P@Lyso could effectively inhibit the proliferation of methicillin-resistant Staphylococcus aureus and accelerated wound disinfection of mice with negligible biological toxicities. The outstanding antibacterial activities of M@P@Lyso were attributed to the photo-enhanced lysozyme activity, assisted by bacterial death caused by the mild local hyperthermia and the physical destruction derived from the M@PDA. This work exemplified a solution to the bacterial resistance threats via stimuli-responsive enzymatic nanoplatforms.
Zhang et al. (Wed,) studied this question.