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The systematic characterization of gene functions in species recalcitrant to Agrobacterium -based transformation, like Pisum sativum , remains a challenge. To develop a high throughput forward and reverse genetics tool in pea, we have constructed a reference ethylmethane sulfonate mutant population and developed a database, UTILLdb, that contains phenotypic as well as sequence information on mutant genes. UTILLdb can be searched online for TILLING alleles, through the BLAST tool, or for phenotypic information about mutants by keywords.
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Dalmais et al. (2008) studied this question.
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