Seroprevalence Rate and Factors Causing African swine fever Outbreak in Kupang City and Regency of East Nusa Tenggara Province, Indonesia

African Swine Faver (ASF) disease was reported in Indonesia in 2019. ASF outbreaks occurred in East Nusa Tenggara Province in 2020 and 2021, with hundreds of thousands of pig deaths. An effective vaccine to prevent the transmission of ASF is currently undiscovered. The diagnosis of ASF is based on the observation of clinical symptoms, observation of the epidemiology of the disease, and laboratory examinations of both serological, virological, and post- mortem tests. Rapid and accurate serology tests are indispensable to prevent the transmission of ASF infection. A risk- based approach to infectious sources needs to be taken to prevent the emergence of ASF outbreaks. This study aims to determine ASF seroprevalence and identify factors that play a role in the transmission of the ASF virus. The results of the study obtained a seroprevalence rate of 52.9%. Risk factors that act as a source of ASF transmission are the origin of pig livestock from the animal market (6.7%), feed from swill feeding (61.7%), non-routine health checks carried out (90%), pig carcasses thrown into landfills (20%), manure waste left alone (33.3%) and sick pigs immediately sold (60%).