Defining Substrate Specificities of N-Acetylglucosamine-6-O-Sulfotransferases for Enzymatic Modular Assembly of Sulfated O-Glycans

Shuquan Fan; Jinghua Han; Tangliang Shen; Mohammadhossein Shabahang; Zhenming Du; Jun pan; Guitao Bai; Shumin Bao; Avinash Ittuveetil; Lei Li

doi:10.1039/d6qo00226a

January 1, 2026Organic Chemistry FrontiersOpen Access

Defining Substrate Specificities of N-Acetylglucosamine-6-O-Sulfotransferases for Enzymatic Modular Assembly of Sulfated O-Glycans

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Key Points

The research aims to clarify how N-acetylglucosamine-6-O-sulfotransferases determine the specific ways sulfated O-glycans are formed.
Analyzed substrate specificities of N-acetylglucosamine-6-O-sulfotransferases.
Examined enzymatic reactions to assess regioselectivity in sulfation.
Utilized biochemical assays to test the efficacy of sulfotransferases.
Identified specific patterns of substrate recognition by sulfotransferases.
Demonstrated improved regioselective sulfation for targeted O-glycan synthesis.
Highlighted the potential for enhanced glycan engineering through defined enzyme specificities.

Implication

Research defines substrate specificities of sulfotransferases for efficient O-glycan synthesis, suggesting improvements for glycan engineering.

Abstract

Sulfated O-glycans are widely distributed and play key roles in a wide range of physiological and disease processes, yet their synthesis remains challenging due to difficulties in regioselective sulfation. Here,...

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Cite This Study

Fan et al. (2026) studied this question.

synapsesocial.com/papers/69df2c50e4eeef8a2a6b1549 https://doi.org/10.1039/d6qo00226a

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Defining Substrate Specificities of N-Acetylglucosamine-6-O-Sulfotransferases for Enzymatic Modular Assembly of Sulfated O-Glycans

Key Points

Implication

Abstract

Discussion

Cite This Study

Defining Substrate Specificities of N-Acetylglucosamine-6-O-Sulfotransferases for Enzymatic Modular Assembly of Sulfated O-Glycans

Key Points

Implication

Abstract

Discussion

Cite This Study

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