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April 15, 2026Journal of Chromatographic Science

Analytical quality by design (AQbD)-assisted bioanalytical method for the estimation of Lasmiditan in human plasma by reverse phase–high performance liquid chromatography (RP-HPLC)

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Authors

AAAjitha AzhakesanBSBurugula SparshaPKPasupuleti Kishore

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Overview

Analytical method estimates Lasmiditan in human plasma, indicating enhanced accuracy and precision for clinical applications.

Key Points

  • The aim is to develop and validate a bioanalytical method for estimating Lasmiditan in human plasma using RP-HPLC and AQbD principles.
  • Utilized Reverse Phase-High Performance Liquid Chromatography for analysis.
  • Employed protein precipitation to extract Lasmiditan using Dolutegravir.
  • Applied Design of Experiments to optimize critical analytical attributes.
  • Conducted validations focusing on linearity, accuracy, and precision.
  • Assessed method efficiency using the Whiteness Assessment Tool.
  • Established linearity for Lasmiditan between 20 and 800 ng/mL (r2 = 0.999).
  • Achieved an accuracy recovery of 98.79%.
  • Received a high performance score of 82.5 on the Whiteness Assessment Tool, indicating superior analytical efficiency.

Cite This Study

Azhakesan et al. (2026) studied this question.

synapsesocial.com/papers/69df2cb9e4eeef8a2a6b1e7fhttps://doi.org/10.1093/chromsci/bmag012
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