Glucose sensor based on an electrochemical reduced graphene oxide-poly(l-lysine) composite film modified GC electrode

A convenient and environmentally friendly method of fabricating glucose biosensors is proposed. Glucose oxidase (GOD) was immobilized on electrochemically reduced graphene oxide (ERGO) which was adsorbed on the poly-L-lysine (PLL) modified glassy carbon electrode after being immersed in GO solution for 4 h. The electrochemical behaviors of GOD/ERGO/PLL/GC electrode have been investigated by cyclic voltammetry. Direct electron transfer between GOD immobilized with ERGO/PLL and GC electrode was observed. Moreover, the GOD/ERGO/PLL/GC electrode exhibited excellent electrocatalytic activity for the detection of glucose with a linear range from 0.25 to 5 mmol L(-1).