Potential protein-encoded synthesis of DNA and RNA

As stated by the central dogma of molecular biology, in living organisms the sequence information of DNA is trans- ferred to RNA and then to protein, but such information cannot be transferred back from protein to nucleic acids. In this article, it is proposed that the sequence in- formation encoded by protein can be arti- ficially transferred back to DNA and RNA, respectively, based on transcription activa- tor-like effectors (TALE) and Pumilio/fem-3 mRNA-binding factors (PUF). Specifically, mono- and/or dinucleotides are assumed to be arranged along the characteristic amino acids of TALE and PUF, and then assembled as oligonucleotides by ligase or condensation agents. This hypothesis suggests a new protein-based strategy for synthesizing DNA and RNA molecules. INTRODUCTION The central dogma of molecular biology, as proposed by Francis Crick in 1958 1 5,6,7,8 . In this article, I suggest the possibility that the sequence information encoded by protein can be artificially transferred resi - due by residue directly to DNA and RNA, respectively, based on transcription ac- tivator-like effectors (TALE) and Pumilio/ fem-3 mRNA-binding factors (PUF). This hypothesis, if proved experimentally, sug- gests a new strategy for the synthesis of short DNA/RNA molecules.