Phytochemical investigation on the 75% EtOH fraction of the green walnut husks of Juglans mandshurica Maxim led to the isolation of four new naphthalene glycosides, Juglophenoside I (1), Juglophenoside II (2), Juglanoside R (3), juglanbioside F (4) as well as 13 known naphthalene compounds (5-17). Structural elucidation of all compounds was carried out by spectroscopic methods such as 1D and 2D NMR spectroscopy (1H-1HCOSY, HSQC, and HMBC), and high resolution mass spectrometry. Moreover the compounds were evaluated for their cytotoxic activities against three cancer cell lines BGC-823, Hela, and HepG-2. Results showed that compounds 1 and 13 had inhibitory effects on HeLa, BGC-823 and Hep-G2 cells, with IC50 values of (58.57 ± 0.74), (62.83 ± 0.91), (68.14 ± 0.72) µM and (61.17 ± 1.74), (67.24 ± 2.24), (56.35 ± 0.55) µM.
Sun et al. (Wed,) studied this question.