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April 18, 2026MicroorganismsOpen Access

High-Level Production of NMN in Escherichia coli Through Co-Utilization of Glucose and Glycerol

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Authors

JGJiajia GanXCX ChenYHYongzhi He

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Overview

Engineered microorganisms increase NMN production in bacteria, suggesting a scalable production method.

Key Points

  • The study aims to enhance the production of nicotinamide mononucleotide (NMN) using engineered E. coli strains.
  • Engineered NMN-producing strain NMN008 to co-utilize glucose and glycerol.
  • Deleted glycolytic genes pgi and pykA/pykF to disrupt glucose catabolism.
  • Introduced a feedback-resistant glycerol kinase mutant (glpK*) to improve glycerol utilization.
  • Conducted experiments in a 2 L bioreactor to evaluate NMN production.
  • Achieved an NMN titer of 32.92 g/L.
  • Increased NMN production by 26.28%.
  • Improved carbon conversion efficiency by 34.48%.

Cite This Study

Gan et al. (2026) studied this question.

synapsesocial.com/papers/69e3213840886becb654062bhttps://doi.org/10.3390/microorganisms14040897
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