Sensitive Electrochemical Analysis of Gentamicin Sulfate in Eye Solution

In this study, for the first time, chromium (III) oxide‐glutardialdehyde modified boron‐doped diamond electrode (Cr 2 O 3 ‐GA 2 /BDDE) was used as a sensor for the electrochemical analysis of gentamicin sulfate. The Cr 2 O 3 ‐GA 2 suspension used in electrode modification was physically coated on the BDD electrode by a simple drop‐casting method. Characterization of Cr 2 O 3 ‐GA 2 used in the modification was made by scanning electron microscopy, energy‐dispersive spectroscopy (SEM‐EDS), and the electrochemical method. The behavior of gentamicin sulfate on Cr 2 O 3 ‐GA 2 /BDDE was studied by cyclic voltammetry (CV), and its electrochemical analysis was done by differential pulse voltammetry (DPV). Electrochemical analysis of gentamicin sulfate on Cr 2 O 3 ‐GA 2 /BDDE was carried out in a pH 9 Britton–Robinson (BR) buffer by the DPV method. The linear working range of gentamicin sulfate on Cr 2 O 3 ‐GA 2 /BDDE was calculated as 5.96 × 10 −6 −6.54 × 10 −5 mol L −1 , and the correlation coefficient was calculated as 0.997. The limit of detection (LOD) was found to be 2.84 × 10 −6. mol L −1 . Additionally, Genta, a 3% eye/ear drop solution, was preferred to determine the applicability of gentamicin sulfate in a commercial formulation. As a result, it was shown that the method developed on Cr 2 O 3 ‐GA 2 /BDDE for the determination of gentamicin sulfate can be applied to commercial drugs.