Quantum Dot-Phage Nanoarchitectonics for Targeted Synergistic Therapy of Methicillin-Resistant Staphylococcus aureus Wound Infections

Chronic wound infections caused by methicillin-resistant Staphylococcus aureus (MRSA) often refractory to conventional therapies due to bacterial drug resistance and biofilm formation. To address this challenge, we developed a synergistic antibacterial nanoarchitectonics integrating bacteriophages and quantum dots (QDs-Phage). This system was constructed by bioconjugating MRSA-specific bacteriophage with highly photothermally efficient PbS quantum dots, creating an intelligent therapeutic platform that operates on a "target-first, activate-later" principle. Under near-infrared laser irradiation, the nanoarchitectonics exhibited significant synergistic bactericidal activity against both MRSA and its biofilms in vitro. In a murine model of full-thickness skin wounds infected with MRSA, topical application of QDs-Phage combined with light irradiation significantly accelerated wound closure and promoted tissue repair, without inducing obvious systemic toxicity. This study provides a novel strategy for developing localized antibacterial approaches that combine precise targeting with efficient physical bactericidal functions.